![]() If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would beġ00 µg/µL × #1/160000# = 6. The dilution factor after four dilutions is ![]() Repeat the process until you have four tubes. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. serial dilution synonyms, serial dilution pronunciation, serial dilution translation, English dictionary definition of serial dilution. You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. So you multiply each successive dilution by the dilution factor. Serial dilution definition Serial dilution in microbiology refers to a technique used to prepare a series of diluted solutions of a microbial sample, starting from a known concentration, in order to determine the number of viable microorganisms in the original sample. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. If you did the above dilution four times, what would be the final dilution factor? What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent? ![]() The dilution factor or the dilution is the initial volume divided by the final volume.įor example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can be either distilled water or 0.9 saline solution. In serial dilutions, you multiply the dilution factors for each step. ![]() The number (concentration) of viable microbial organisms is estimated from a single dilution plate (assay) without a need for replicate plates. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony. ![]()
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